副猪嗜血杆菌感染:宿主免疫应答模型与巨噬细胞活化

doi:10.11843/j.issn.0366-6964.2015.01.001

摘要/Abstract

摘要：

副猪嗜血杆菌是当前世界养猪业中最主要的细菌性病原体之一，感染后临床表现以格拉泽病为典型特征，严重威胁着猪群健康，应用层面的防治工作仍存在很多问题。近年来，该细菌毒力因子与宿主免疫应答等相关研究取得了很大进步，但基础层面宿主-病原互作的分子机制仍不清楚。基于不同猪对该细菌存在遗传抗性差异，随着猪基因组科学技术的飞速发展，越来越多的国内外学者正寄希望于分子抗病育种途径以从根本上解决细菌感染。为此，本文结合作者所在课题组的以往工作通过综述现有高通量数据初步提出了宿主免疫应答的分子模型，重点探讨巨噬细胞活化对细菌感染或宿主保护性免疫应答可能造成的影响，围绕其中关键的分子调控进行了较为深入的分析，旨在为将来发掘具有应用价值的候选基因素材/分子标记提供借鉴。最后，本综述就下一步相关工作的开展提出了几点思考。

Abstract:

Haemophilus parasuis(HPS) is one of the main virulent bacteria that threating the pig industry worldwide.HPS infection typically causes serious inflammations，namely Glässer’s disease.But there are still many troubles in disease prevention and control.Though much progress has been made in the basic research fields of bacterial virulent factors and host immune responses(IR) recently，the mechanisms of host-bacillus interaction are largely unknown.As the different susceptibilities in pigs exist，genomic selection based on molecular breeding strategy will receiving increasing attention from scientists and commercial breeders.Here，some high through-out data from our and other labs are reviewed in brief and the model for the host IR is proposed.We hypothesize that the activation of macrophage during the early stage of IR may play pivotal roles in determining bacillus escape and/or host protective IRs.In addition，key molecules and pathways involved in the IR of macrophage are discussed，which we hope will be helpful for the marker identifications in objectives of molecular breeding.Still，there is much work to be done and some suggestions are proposed in the article.

中图分类号:

S852.61

陈洪波. 副猪嗜血杆菌感染:宿主免疫应答模型与巨噬细胞活化[J]. 畜牧兽医学报, 2015, 46(1): 1-11.

CHEN Hong-bo. Haemophilus parasuis Infection：Model for the Host Immune Response and the Macrophage Activation[J]. ACTA VETERINARIA ET ZOOTECHNICA SINICA, 2015, 46(1): 1-11.

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参考文献

［1］OLIVEIRA S，PIJOAN C.Haemophilus parasuis：new trends on diagnosis，epidemiology and control［J］.Vet Microbiol，2004，99(1):1-12.
［2］NEDBALCOVA K， SATRAN P， JAGLIC Z， et al.Haemophilus parasuis and Glasser’s disease in pigs：a review［J］.Vet Med，2006，51:168-179.
［3］FABLET C，MAROIS C，KUNTZ-SIMON G，et al.Longitudinal study of respiratory infection patterns of breeding sows in five farrow-to-finish herds［J］.Vet Microbiol，2011，147(3-4):329-339.
［4］YU J， WU J， ZHANG Y， et al.Concurrent highly pathogenic porcine reproductive and respiratory syndrome virus infection accelerates Haemophilus parasuis infection in conventional pigs［J］.Vet Microbiol， 2012，158(3-4):316-321.
［5］KARG G， BILKEI G.The effect of season and vaccination for Glässer’s disease and post-weaning Colibacillosis in an outdoor pig unit endemically infected with virulent strain of Haemophilus parasuis serotype 5 and pathogenic Escherichia coli［J］.J Vet Med B Infect Dis Vet Public Health，2002，49(10):464-468.
［6］XU Z， YUE M， ZHOU R，et al.Genomic characterization of Haemophilus parasuis SH0165，a highly virulent strain of serovar 5 prevalent in China［J］.PLoS One，2011，6(5):e19631.
［7］NUTSCH R G， HART F J， ROONEY K A， et al.Efficacy of tulathromycin injectable solution for the treatment of naturally occurring swine respiratory disease［J］.Vet Ther，2005，6(2):214-224.
［8］VILALTA C，GALOFRÉ N，ARAGON V，et al.Effect of marbofloxacin on Haemophilus parasuis nasal carriage［J］.Vet Microbiol，2012，159(1-2):123-129.
［9］MACEDO N， ROVIRA A， OLIVEIRA S， et al.Effect of enrofloxacin in the carrier stage of Haemophilus parasuis in naturally colonized pigs［J］.Can J Vet Res，2014，78(1):17-22.
［10］BAK H， RIISING H J.Protection of vaccinated pigs against experimental infections with homologous and heterologous Haemophilus parasuis［J］.Vet Rec，2002，151(17):502-505.
［11］BAUMANN G， BILKEI G.Effect of vaccinating sows and their piglets on the development of Glässer’s disease induced by a virulent strain of Haemophilus parasuis serovar 5［J］.Vet Rec，2002，151(1):18-21.
［12］TEIXEIRA M L， DALLA R A， BRANDELLI A.Characterization of an antimicrobial peptide produced by Bacillus subtilis subsp.spizezinii showing inhibitory activity towards Haemophilus parasuis［J］.Microbiology，2013，159(Pt5):980-988.
［13］HU H， WANG C，GUO X， et al.Broad activity against porcine bacterial pathogens displayed by two insect antimicrobial peptides moricin and cecropin B［J］.Mol Cells，2013，35(2):106-114.
［14］MELLENCAMP M A， GALINA-PANTOJA L， GLADNEY C D，et al.Improving pig health through genomics：a view from the industry［J］.Dev Biol(Basel)，2008，132:35-41.
［15］BISHOP S C， LUNNEY J K， PINARD-VAN DER LAAN M H， et al.Report from the second international symposium on animal genomics for animal health：critical needs，challenges and potential solutions［J/OL］.BMC Proc，2011，5(Suppl4):S1.［2014-12-09］.http://www.biomedcentral.com/1753-6561/5/S4/S1.
［16］BLANCO I， CANALS A， EVANS G， et al.Differences in susceptibility to Haemophilus parasuis infection in pigs［J］.Can J Vet Res，2008，72(3):228-235.
［17］CHEN H， LI C， FANG M， et al.Understanding Haemophilus parasuis infection in porcine spleen through a transcriptomics approach［J/OL］.BMC Genomics，2009，10:64.［2014-12-09］.http://www.biomedcentral.com/1471-2164/10/64.
［18］WILKINSON J M， SARGENT C A，GALINA-PANTOJA L，et al.Gene expression profiling in the lungs of pigs with different susceptibilities to Glässer’s disease［J/OL］.BMC Genomics，2010，11:455.［2014-12-09］.http://www.biomedcentral.com/1471-2164/11/455.
［19］WANG Y， LIU C， FANG Y， et al.Transcription analysis on response of porcine alveolar macrophages to Haemophilus parasuis［J/OL］.BMC Genomics，2012，13:68.［2014-12-09］.http://www.biomedcentral.com/1471-2164/13/68.
［20］ZHANG B， HE Y， XU C， et al.Cytolethal distending toxin(CDT) of the Haemophilus parasuis SC096 strain contributes to serum resistance and adherence to and invasion of PK-15 and PUVEC cells［J］.Vet Microbiol，2012，157(1-2):237-242.
［21］ZHANG B， XU C， LIAO M.Outer membrane protein P2 of the Haemophilus parasuis SC096 strain contributes to adherence to porcine alveolar macrophages cells［J］.Vet Microbiol，2012，158(1-2):226-227.
［22］CHEN H， LUNEEY J K， CHENG L， et al.Porcine S100A8 and S100A9：molecular characterizations and crucial functions in response to Haemophilus parasuis infection［J］.Dev Comp Immunol，2011，35(4):490-500.
［23］CHEN Y， JIN H， CHEN P， et al.Haemophilus parasuis infection activates the NF-κB pathway in PK-15 cells through IκB degradation［J］.Vet Microbiol，2012，160(1-2):259-263.
［24］FOELL D， WITTKOWSKI H， ROTH J.Mechanisms of disease：a 'DAMP' view of inflammatory arthritis［J］.Nat Clin Pract Rheumatol，2007，3(7):382-390.
［25］BISWAS S K， LOPEZ-COLLAZO E.Endotoxin tolerance：new mechanisms，molecules and clinical significance［J］.Trends Immunol，2009，30(10):475-487.
［26］DE WAAL MALEFYT R， HAANEN J， SPITS H，et al.Interleukin 10(IL-10) and viral IL-10 strongly reduce antigen-specific human T cell proliferation by diminishing the antigen-presenting capacity of monocytes via downregulation of class II major histocompatibility complex expression［J］.J Exp Med，1991，174(4):915-924.
［27］FIORENTINO D F，ZLOTNIK A，VIEIRA P，et al.IL-10 acts on the antigen-presenting cell to inhibit cytokine production by Th1 cells［J］.J Immunol，1991，146(10):3444-3451.
［28］KARP C L，MURRAY P J.Non-canonical alternatives：what a macrophage is 4［J］.J Exp Med，2012，209(3):427-431.
［29］GORDON S，MARTINEZ F O.Alternative activation of macrophages：mechanism and functions［J］.Immunity，2010，32(5):593-604.
［30］BHATTACHARJEE A，SHUKLA M，YAKUBENKO V P，et al.IL-4 and IL-13 employ discrete signaling pathways for target gene expression in alternatively activated monocytes/macrophages［J/OL］.Free Radic Biol Med，2013，54:1-16.［2014-12-09］.http://www.sciencedirect.com/science/article/pii/S0891584912017959.
［31］SHIREY K A， COLE L E，KEEGAN A D，et al.Francisella tularensis live vaccine strain induces macrophage alternative activation as a survival mechanism［J］.J Immunol，2008，181(6):4159-4167.
［32］KYROVA K， STEPANOVA H，RYCHLIK I， et al.SPI-1 encoded genes of Salmonella Typhimurium influence differential polarization of porcine alveolar macrophages in vitro［J/OL］.BMC Vet Res，2012，8:115.［2014-12-09］.http://www.biomedcentral.com/1746-6148/8/115.
［33］吴媛媛，李龙，沈萍萍.巨噬细胞替代激活及调控［J］.中国细胞生物学学报，2011，33（2）:197-203.
WU Y Y，LI L，SHEN P P.Progress of studies on macrophage alternative activation and its regulation mechanisms［J］.Chinese Journal of Cell Biology，2011，33（2）:197-203.(in Chinese)
［34］PAMPUSCH M S，BENNAARS A M，HARSCH S，et al.Inducible nitric oxide synthase expression in porcine immune cells［J］.Vet Immunol Immunopathol，1998，61(2-4):279-289.
［35］SEGALÉS J，DOMINGO M，BALASCH M，et al.Ultrastructural study of porcine alveolar macrophages infected in vitro with porcine reproductive and respiratory syndrome(PRRS) virus，with and without Haemophilus parasuis［J］. J Comp Pathol，1998，118(3):231-243.
［36］MÜLLER G，KÖHLER H，DILLER R，et al.Influences of naturally occurring and experimentally induced porcine pneumonia on blood parameters［J］.Res Vet Sci，2003，74(1):23-30.
［37］DE LA FUENTE A J，GUTIÉRREZ-MARTÍN C B，RODRÍGUEZ-BARBOSA J I，et al.Blood cellular immune response in pigs immunized and challenged with Haemophilus parasuis［J］.Res Vet Sci，2009，86(2):230-234.
［38］FRANDOLOSO R，MARTÍNEZ-MARTÍNEZ S，YUBERO S，et al.New insights in cellular immune response in colostrum-deprived pigs after immunization with subunit and commercial vaccines against Glässer’s disease［J］.Cell Immunol，2012，277(1-2)：74-82.
［39］COSTA-HURTADO M，OLVERA A，MARTINEZ-MOLINER V，et al.Changes in macrophage phenotype after infection of pigs with Haemophilus parasuis strains with different levels of virulence［J］.Infect Immun，2013，81(7):2327-2333.
［40］RILEY J， TAKEDA K， AKIRA S， et al.Interleukin-10 receptor signaling through the JAK-STAT pathway.Requirement for two distinct receptor-derived signals for anti-inflammatory action［J］.J Biol Chem，1999，274(23):16513-16521.
［41］PRADEL L C，MITCHELL A J，ZARUBICA A，et al.ATP-binding cassette transporter hallmarks tissue macrophages and modulates cytokine-triggered polarizationprograms［J］.Eur J Immunol，2009，39(8):2270-2280.
［42］TANG C， LIU Y， KESSLER P S，et al.The macrophage cholesterol exporter ABCA1 functions as an anti-inflammatory receptor［J］.J Biol Chem，2009，284(47):32336-32343.
［43］MA L， DONG F， ZAID M， et al.ABCA1 protein enhances Toll-like receptor 4(TLR4)-stimulated interleukin-10(IL-10) secretion through protein kinase A(PKA) activation［J］.J Biol Chem，2012，287(48):40502-40512.
［44］ZHU X， WESTCOTT M M，BI X， et al.Myeloid cell-specific ABCA1 deletion protects mice from bacterial infection［J］.Circ Res，2012，111(11):1398-1409.
［45］ZHANG B， TANG C， LIAO M， et al.Update on the pathogenesis of Haemophilus parasuis infection and virulence factors［J］.Vet Microbiol，2014，168(1):1-7.
［46］COSTA-HURTADO M，ARAGON V.Advances in the quest for virulence factors of Haemophilus parasuis ［J］.Vet J，2013，198(3):571-576.
［47］BLACKALL P J， TURNI C.Understanding the virulence of Haemophilus parasuis［J］. Vet J，2013，198(3):549-550.
［48］OLIVEIRA S， GALINA L， BLANCO I，et al.Naturally-farrowed，artificially-reared pigs as an alternative model for experimental infection by Haemophilus parasuis［J］.Can J Vet Res，2003，67(2):146-150.
［49］BLANCO I， GALINA-PANTOJA L， OLIVEIRA S， et al.Comparison between Haemophilus parasuis infection in colostrums-deprived and sow-reared piglets［J］.Vet Microbiol，2004，103(1-2):21-27.

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